Hemoperfusion alleviated paraquat-induced kidney inflammation injury of rabbit.
- Author:
Shaoce ZHI
1
;
Dong WU
1
;
Lingwen LI
1
;
Guangliang HONG
1
;
Mengfang LI
1
;
Bin WU
1
;
Zhongqiu LU
2
;
E-mail: LZQ640815@163.COM.
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hemoperfusion; Inflammation; chemically induced; therapy; Interleukin-6; metabolism; Kidney; drug effects; physiopathology; Male; NF-kappa B; metabolism; Nitric Oxide Synthase Type II; metabolism; Paraquat; toxicity; RNA, Messenger; metabolism; Rabbits; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(9):658-662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hemoperfusion on paraquat-Induced kidney inflammation injury of rabbit and the mechanism of it.
METHODS60 male rabbits were randomly divided into 4 groups, the normal control group (n=6, the rabbits were given NS by gavage) , blank control group (n=18, he rabbits were given 2 hours hemoperfusion once within 1 hour after given NS by gavage), paraquat poisoning group (n=18, the rabbits were given 50 mg/kg 20% paraquat solution by gavage) , hemoperfusion treatment group (n=18, the rabbits were given 2 hours hemoperfusion once within 1 hour after 20% paraquat solution espoused). The last 3 groups were divided into 3 observation time groups (1, 3, 7 day), contained 6 rabbits each group. On days 1, 3, 7 all groups rabbits were anesthetized and sacrificed, and their kidney tissues collected. The levels of NF-κB mRNA by RT-PCR, and the expression of NF-κB protein was measured by Western blotting,The expression levels of TNF-α, IL-6, iNOS measured by chemical colorimetric method to to observe inflammatory injury.
RESULTSCompared with the normal control group rabbits, there were no changes in the TNF-α, IL-6, iNOS, NF-κB mRNA and protein of blank control group (P>0.05), while the expression of TNF-α, IL-6, NF-κB mRNA and protein in the kidney tissue of PQ group and were significantly increased (P<0.05). The pathological results of kidney tissues were no abnormalities onnormal control group and blank control group.
CONCLUSIONHP significantly increase resistance to PQ-induced inflammation injury in the rabbit kidney and exert a protective effect on PQ-induced kidney injury.