In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells.
- Author:
Jia-ye HUA
1
;
Ren-wei HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens; immunology; Cytotoxicity, Immunologic; immunology; Dendritic Cells; drug effects; immunology; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Humans; Interleukin-4; pharmacology; K562 Cells; L-Lactate Dehydrogenase; metabolism; Lymphocyte Activation; immunology; Lymphocyte Culture Test, Mixed; T-Lymphocytes, Cytotoxic; enzymology; immunology; Tumor Necrosis Factor-alpha; pharmacology
- From: Journal of Southern Medical University 2006;26(5):617-619
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of dendritic cells (DC) stimulated with K562 cell lysate in inducing specific cytotoxic T lymphocytes (CTL) against K562 cells in vitro.
METHODSThe DCs were derived from healthy human peripheral blood monocytes in the presence of granulocyte-macrophage colony-stimulating factor, interleukin (IL)-4 and tumor necrosis factor (TNF) alpha. The T cells were stimulated by DCs loaded with freeze-thawed K562 cells and T-cell cytotoxicities were measured by lactate dehydrogenase (LDH) assay.
RESULTSThe DCs could be successfully obtained from peripheral blood monocyte after the culture. Mixed lymphocyte reactions induced by the antigen-loaded DC were much stronger than those induced by human peripheral blood monocytes (P<0.05). At the effector to target ratio of 10:1 and 20:1, cytotoxicities against K562 cells by CTL derived from culture with the antigen-loaded DCs were the strongest (P<0.05).
CONCLUSIONCTL derived from DCs pulsed with K562 cell lysate show effective and specific cytotoxicity against K562 cells.
