Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line.
- Author:
Chun HAN
1
;
Shuang WANG
;
Li LIU
;
Kai-tai YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Squamous Cell; genetics; metabolism; secondary; Cell Line, Tumor; Cell Proliferation; Cell Survival; Disease Models, Animal; Female; Green Fluorescent Proteins; genetics; metabolism; Humans; Keratins; analysis; Liver Neoplasms, Experimental; genetics; metabolism; secondary; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Nasopharyngeal Neoplasms; genetics; metabolism; pathology; Neoplasm Metastasis; Neoplasm Transplantation
- From: Journal of Southern Medical University 2006;26(5):654-657
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a hepatic metastatic subline of nasopharyngeal carcinoma (NPC) cell line.
METHODSNPC cells metastatic to the liver were isolated from nude mice and the invasion and metastatic ability of the cells was observed in vivo and in vitro.
RESULTS AND CONCLUSIONThe invasion and metastasis activity of 5-8F-H3B-EGFP (an in vivo isolate with enhanced liver metastatic behaviors) were enhanced obviously in comparison with the parent cell line 5-8F-EGFP. This subline may be useful for cloning genes related to liver metastasis of NPC.
