Effects of motilin agonists on intracellular calcium mobilization in cultured rat myenteric neurons.
- Author:
Xia YANG
1
;
Lei DONG
;
Xi-en YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcium; metabolism; Calcium Signaling; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Erythromycin; pharmacology; Microscopy, Confocal; Motilin; pharmacology; Myenteric Plexus; cytology; metabolism; Neurons; cytology; drug effects; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Gastrointestinal Hormone; agonists; Receptors, Neuropeptide; agonists
- From: Journal of Southern Medical University 2006;26(6):760-763
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of motilin agonists on intracellular Ca(2+) mobilization in primary cultured rat myenteric neurons.
METHODSMotilin-induced and erythromycin-induced intracellular Ca(2+) signaling was studied in primary cultures of rat myenteric neurons using the radiometric Ca(2+) indicator Furo3/AM with a laser confocal microscope.
RESULTSIn Hank's solution, 10(-8), 10(-7), and 10(-6) mol/L motilin could elevate intracellular Ca(2+) concentration ([Ca(2+)]i) to the peak levels of 10.6-/+2.1, 15.9-/+1.2, and 30.6-/+3.7 respectively with their relative percentage change in fluorescent intensity of (40.1-/+6.3)%, (63.0-/+11.2)%, and (100.8-/+18.4)% respectively, indicating the dose-dependent effect of motilin on [Ca(2+)]i. In Hank's solution, 10 microg/ml erythromycin could induce the elevation of [Ca(2+)]i to the average peak of 23.2-/+5.6 with the relative percentage change in fluorescent intensity of (82.8-/+13.0)%. When pretreated with the antibody against motilin receptor in Hank's solution, the effect of 10 microg/ml erythromycin was almost inhibited completely.
CONCLUSIONMotilin can increase [Ca(2+)]i, and erythromycin also has this effect by binding to motilin receptor.
