Ganoderma polysaccharides antagonize prostaglandin E2-induced suppression of murine splenocyte IFN-gamma and TNF-alpha mRNA expression.
- Author:
Qun ZHANG
1
;
Lin-sheng LEI
;
Zheng-guang ZHU
;
Chuan-lin YU
;
Shu-guang WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Dinoprostone; pharmacology; Female; Gene Expression; drug effects; Interferon-gamma; genetics; Lymphocytes; cytology; drug effects; metabolism; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Polysaccharides; pharmacology; RNA, Messenger; biosynthesis; genetics; Reishi; chemistry; Reverse Transcriptase Polymerase Chain Reaction; Spleen; cytology; Tumor Necrosis Factor-alpha; genetics
- From: Journal of Southern Medical University 2006;26(6):780-783
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine if Ganoderma polysaccharides can antagonize prostaglandin E2 (PGE2)-induced suppression of murine splenocyte interferongamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) mRNA expression.
METHODSMixed lymphocyte culture reaction was used as the experimental model. The expressions levels of IFN-gamma and TNF-alpha mRNA were measured by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSAfter the cultures were treated with PGE2 for 4 h, IFN-gamma mRNA expression was reduced as compared with the control, which was especially obvious when PGE2 concentrations exceeded 10 micromol/L (P<0.01). Ganoderma polysaccharides above 100 mg/L showed partial antagonistic effect against the inhibition of IFN-gamma by PGE2 at the fixed concentration of 20 micromol/L. Further studies indicated that PGE2 (20 micromol/L) impaired the expression of TNF-alpha mRNA after an 8-hour incubation and Ganoderma polysaccharides above 100 mg/L could partially antagonize this effect.
CONCLUSIONGanoderma polysaccharides can partially antagonize PGE2-induced suppression of murine splenocyte IFN-gamma and TNF-alpha mRNA expression.
