Cloning of A73 gene and its coding sequence analysis.
- Author:
Ang LI
1
;
Song ZHAO
;
Wei HU
;
Gao-feng ZHAO
;
Xiao-shi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Molecular; DNA, Complementary; chemistry; genetics; Herpesvirus 4, Human; genetics; Humans; Molecular Sequence Data; Nasopharyngeal Neoplasms; pathology; virology; Open Reading Frames; genetics; Point Mutation; Sequence Analysis, DNA; Viral Proteins; genetics
- From: Journal of Southern Medical University 2006;26(6):826-830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone A73 gene of Epstein-Barr virus (EBV) and examine the variation of its coding sequence.
METHODSA73 coding sequence (CDS) amplified from 7 patients in nasophryngeal carcinoma (NPC) biopsies by RT-PCR was cloned into pGEM-T-Easy vector to construct the recombinant plasmid, which was subjected to sequence analysis in Gen Bank database using Blast software.
RESULTSA73 gene from nasophryngeal carcinoma was successfully cloned into pGEM-T Easy vector. A locus with conversion of A-->C in A73 was found at CDS 45 nt, located in the exonVB157154 nt, which did not result in an amino acid replacement, and the variation frequency was 7/7.
CONCLUSIONThere is a point mutation in A73 CDS of EBV isolated from NPC tissue, which might produce NPC-associated polymorphism with possible involvement in the composition of some subtype of EB virus.
