Co-culture of human extravillous cytotrophoblasts and decidual stromal cells in vitro.
- Author:
Li-yun QIN
1
;
Shi-ling CHEN
;
Xi-qian ZHANG
;
Yan-qiu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Communication; physiology; Cells, Cultured; Chorion; cytology; Coculture Techniques; Decidua; cytology; Female; Humans; Models, Biological; Stromal Cells; cytology; Trophoblasts; cytology; physiology
- From: Journal of Southern Medical University 2006;26(7):914-917
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a convenient method for isolation and purification of human extravillous cytotrophoblasts (EVCTs) and decidual stromal cells (DSCs) and establish a co-culture system.
METHODSThe DSCs were digested with trypsin and purified by Percoll gradient. The EVCTs were digested with trypsin and purified by BSA gradient. Immunohischemistry and immunofluorescent study are performed to characterize these isolated cells. The EVCTs and DSCs were placed in Matrigel-coated Transwell upper and lower chamber, respectively, to study the invasive ability of the EVCTs.
RESULTSImmunohischemistry revealed that the purity of EVCTs and DSC exceeded 95%. Cultured EVCTs retained their capacity to invade Matrigel-coated Transwell filters with the invasion index of 3.22-/+0.04.
CONCLUSIONThis co-culture model established by isolating highly purified EVCTs and DSCs in vitro can be useful for studying the trophoblast invasion mechanisms.
