Effects of proadrenomedullin N-terminal 20 peptide on angiotensin II-induced NO production in rat cardiac fibroblasts.
- Author:
Shi-rong XUE
1
;
Zhi-liang LI
;
Chun-sheng XU
;
Quan-eng YAN
;
Hai-long JIANG
;
Hong-chao WU
;
Chao-shu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenomedullin; Angiotensin II; pharmacology; Animals; Animals, Newborn; Cells, Cultured; Fibroblasts; cytology; drug effects; metabolism; Myocytes, Cardiac; cytology; drug effects; metabolism; Nitric Oxide; biosynthesis; Peptide Fragments; pharmacology; Protein Precursors; chemistry; pharmacology; Proteins; chemistry; pharmacology; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2006;26(7):936-938
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of proadrenomedullin N-terminal 20 peptide (PAMP) on nitric oxide (NO) production in rat cardiac fibroblasts (CFs) induced by angiotensin II (AngII) stimulation.
METHODSNeonatal SD rat CFs isolated by trypsin digestion were cultured and stimulated with PAMP, AngII or their combination, and NO production in the CFs in response to the treatments was measured by nitric acid reductase method.
RESULTSNO levels in the cell culture treated with 1x10(-9), 1x10(-8), 1x10(-7), and 1x10(-6) micromol/L AngII were 73.88-/+2.23, 64.34-/+3.02, 54.12-/+2.82, and 40.21-/+1.45 micromol/L, respectively, showing significant differences between the groups (P<0.01), whereas treatment of the cells with 1x10(-9), 1x10(-8), 1x10(-7), and 1x10(-6) micromol/L PAMP did not result in significant variation in NO production (74.40-/+3.42, 74.91-/+2.66, 75.77-/+3.31, and 74.23-/+2.43 micromol/L, respectively) in comparison with that of the blank control group (74.57-/+2.49 micromol/L, P>0.05). Combined treatments with 1x10(-7) micromol/L AngII and PAMP at 1x10(-9), 1x10(-8), 1x10(-7), and 1x10(-6) micromol/L PAMP caused significant increment of NO production (66.15-/+2.95, 80.58-/+3.77, 88.67-/+1.46, and 96.22-/+2.96 micromol/L, respectively, P<0.01) in a PAMP dose-dependent manner, suggesting the abolishment of AngII-induced enhancement of NO production in the CFs by PAMP.
CONCLUSIONPAMP increases NO production in the CFs in the presence of AngII but it does not induce significant changes in NO production when used alone.
