An epidemiological investigation of bats carrying SARS-CoV in Guangzhou and its vicinity.
- Author:
Zhi-feng LI
1
;
Yong HU
;
Hui-chun ZHAN
;
Xue-xia YUN
;
Yu-ping DU
;
Xue-mei KE
;
De-xian YU
;
Jian-dong LI
;
Ying-chun DAI
;
Qing CHEN
;
Shou-yi YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; epidemiology; Chiroptera; virology; Disease Vectors; Enzyme-Linked Immunosorbent Assay; Humans; Nucleocapsid Proteins; metabolism; RNA, Viral; genetics; Reverse Transcriptase Polymerase Chain Reaction; SARS Virus; genetics; isolation & purification; metabolism; Severe Acute Respiratory Syndrome; epidemiology; transmission; virology
- From: Journal of Southern Medical University 2006;26(7):949-953
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect serve acute respiratory syndrome-associated coronavirus (SARS-CoV) and SARS-like-CoV in fruit bats captured in Guangzhou and its vicinity.
METHODSTotally 927 bats of 9 species (Cynopterus sphinx, Rousettus leschenaulti, Miniopterus schreibersi, Hipposideros pratti, Rhinolophusasinicus, Scotophilusakuhlii, Hipposideros Pomona, Rhinolophus affinis, and Rhinolophus pusillus) captured in Guangzhou and its vicinity from September 2004 to November 2005 were available for this investigation, from which 3,043 samples (813 throat swasb, 524 sera, 853 lung tissues and 853 colorectal tissue specimens) were obtained. SARS-Cov and SARS-like-CoV were detected in these specimens using diagnostic kit for novel coronavirus N protein (ELISA), SARS-CoV Virus RNA detection kit, fluorescence PCR, Genchip, RT-PCR and cell isolation culture methods.
RESULTS AND CONCLUSIONNo SARS-CoV and SARS-like-CoV were detected in the 3043 samples, indicating the current absence of SARS-CoV and SARS-like-CoV in the bats captured in Guangzhou and its vicinity.