GATA-3 expression in the development of anterior chamber associated immune deviation.
- Author:
Tao FU
1
;
Pei-zeng YANG
;
Xiang-kun HUANG
;
Qiang HUANG
;
Hong-yan ZHOU
;
Bing LI
;
Hua-hong ZHONG
;
Xuan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anterior Chamber; immunology; Eye Proteins; immunology; Female; GATA3 Transcription Factor; analysis; genetics; physiology; Hypersensitivity, Delayed; immunology; RNA, Messenger; analysis; Rats; Rats, Wistar; Retinol-Binding Proteins; immunology; Spleen; metabolism; Th2 Cells; immunology
- From: Chinese Medical Journal 2005;118(23):2000-2004
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDAnterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.
METHODSACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTSA significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.
CONCLUSIONThe expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.
