Migration and distribution of allogeneic T lymphocytes in organs of graft-versus-host disease mouse model.
- Author:
Hong-Sheng WEN
1
;
Jian-Min WANG
;
Hong ZHOU
;
Rong XIA
;
Hui-Ying QIU
;
Lei GAO
;
Xiao-Xia HU
Author Information
1. Department of Hematology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Transplantation;
adverse effects;
Cell Movement;
Female;
Graft vs Host Disease;
immunology;
pathology;
Green Fluorescent Proteins;
Liver;
immunology;
pathology;
Lung;
immunology;
pathology;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Skin;
immunology;
pathology;
Spleen;
cytology;
T-Lymphocytes;
immunology
- From:
Journal of Experimental Hematology
2006;14(5):919-923
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the migration and distribution processes of allogeneic donor T lymphocytes in the organs of recipient mice. GVHD model was established by transfusion of the splenocytes of eGFP transgeneic C57BL/6 mice together with born marrow cells harvested from C57BL/6 mice into BALB/c mice underwent 8.0 Gy total body irradiation. The migration and homing of eGFP(+) cells were tracked by stereo-fluorescent microscopy or inverted fluorescent microscopy and flow cytometry. The enzyme linked immunosorbent assay (ELISA) was performed on supernatants from the tissue homogenates to detect the amount of MIP-1alpha. The results indicated that GVHD clinical manifestation and pathological changes of organs appeared on day 8 post transplantation. eGFP-positive donor T cells in recipient organs were observed by inverted fluorescence microscope in frozen section, or by stereo-fluorescence microscopy in living organs, such as liver, spleen, skin, lungs, bowels, and tongue. The highest expression of MIP-1alpha was on day 7 post transplantation in the liver (491.3 +/- 32.1 pg/ml), and day 3 post transplantation in the spleen (881.5 +/- 45.2 pg/ml), respectively (P < 0.05). It is concluded that GVHD was induced by splenocytes of eGFP transgeneic C57BL/6 mice. eGFP(+) cells in the organs can be observed by fluorescent microscopy. In this GVHD model, donor T cells proliferate and infiltrate in liver, skin, bowels, as well as lungs and tongue. MIP-1alpha may be in relation with the infiltration of T lymphocytes in liver and spleen.
