Synergistic effects of total saponins of panax ginseng in combination with hematopoietic growth factor on proliferation and differentiation of CD34(+) cells ex vivo.
- Author:
Jian-Wei WANG
1
;
Ya-Ping WANG
;
Sha-Li WANG
;
Rong JIANG
Author Information
1. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing University of Medical Sciences, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Drug Synergism;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Hematopoietic Stem Cells;
cytology;
immunology;
Humans;
Panax;
chemistry;
Saponins;
pharmacology
- From:
Journal of Experimental Hematology
2006;14(5):959-963
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of total saponins of panax ginseng (TSPG) in combination with hematopoietic growth factors (HGF) on proliferation and differentiation of CD34(+) cells ex vivo, the purified CD34(+) cells from cord blood and bone marrow were expanded by various concentrations of TSPG with combination of cytokines in liquid culture systems and the expanded cell number, CD34(+) cell number, CD33(+) cell ratio, the numbers of total CFC and hematopoietic progenitor cell number were detected. The results showed that TSPG (10 - 70 microg/ml) could raise the expanded cell number, CD34(+) cell number, and the numbers of total CFC, TSPG 50 microg/ml was identified as the most potent stimulating concentration, and increased total nucleated cells to (2470.5 +/- 79.96) x 10(3), CFC to (53.96 +/- 4.286) x 100% and CD34(+) cells to (21.86 +/- 3.094) x 100%; TSPG (10 - 50 microg/ml) could raise the colony formation rate of CFU-GM, TSPG (20 microg/ml) induced the best effect on granulocytopoietic differentiation committed of CD34(+) cells. It is concluded that the optimal concentration of TSPG can promote CD34(+) cells to proliferate and differentiate by cooperating with hematopoietic growth factors.
