Impact of mobilization with rhG-CSF on the proliferation and cytotoxicity of donor's T cells.
- Author:
Wen-Rong HUANG
1
;
Li-Sheng WANG
;
Chun-Ji GAO
;
Zhuo-Zhuang LU
;
Hua WANG
;
Hai-Feng DUAN
;
Wan-Ming DA
Author Information
1. Department of Hematology, General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Cell Proliferation;
drug effects;
Cells, Cultured;
Fas Ligand Protein;
biosynthesis;
genetics;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor;
administration & dosage;
pharmacology;
Hematopoietic Stem Cell Mobilization;
methods;
Humans;
Male;
Middle Aged;
RNA, Messenger;
biosynthesis;
genetics;
Recombinant Proteins;
T-Lymphocytes;
cytology;
drug effects;
T-Lymphocytes, Cytotoxic;
drug effects;
immunology;
fas Receptor;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2006;14(5):995-998
- CountryChina
- Language:Chinese
-
Abstract:
The study was to understand the impact on the proliferation and cytotoxicity of donor's T cells during mobilization with rhG-CSF. The peripheral blood mononuclear cells (PBMNC) were collected from 15 donors before mobilization and on fifth day of mobilization with rhG-CSF. After the PBMNC were activated with 500 ng/ml of CD3 monoclonal antibody and 500 microg/ml of rhIL-2 for 96 hours, the activated T cells were collected for testing proliferation, cytotoxicity, Fas expression, perforin and Fas ligand (FasL) mRNA expression, the IFN-gamma concentration in the culture medium of the activated T cells was determined by radioimmunoassay. The results showed that the proliferation activity of T lymphocytes and the cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL-2 were reduced markedly after mobilization with rhG-CSF (P < 0.05). The Fas molecule expression in the activated T cells was very high both before and after mobilization with rhG-CSF (P > 0.10). The activated T cells expressed perforin mRNA and didn't express FasL mRNA both before and after mobilization with rhG-CSF. The concentration of IFN-gamma in the culture medium of the activated T cells decreased significantly after mobilization with rhG-CSF (P < 0.01). It is concluded that activity of proliferation and cytotoxicity of donor's T cells is impaired after mobilization with rhG-CSF.
