Hematopoietic growth factors expressed in human aorta-gonad-mesonephros (AGM)-derived stromal cells in vitro.
- Author:
Hui-Qin CHEN
1
;
Xu-Chao ZHANG
;
Xin-Yi TANG
;
Bei-Yan WU
;
Shao-Liang HUANG
Author Information
1. Center for Stem Cell Research, The Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
- Publication Type:Journal Article
- MeSH:
Aorta;
cytology;
embryology;
metabolism;
Cells, Cultured;
Embryonic Stem Cells;
cytology;
metabolism;
Endothelial Cells;
cytology;
metabolism;
Gonads;
cytology;
embryology;
metabolism;
Hematopoiesis;
physiology;
Hematopoietic Stem Cells;
cytology;
Humans;
Interleukin-6;
biosynthesis;
genetics;
Mesonephros;
cytology;
metabolism;
Oncostatin M;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Stromal Cells;
cytology;
metabolism;
fms-Like Tyrosine Kinase 3;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2006;14(5):999-1003
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the hematopoietic growth factors expressed in human aorta-gonad-mesonephros (AGM)-derived stromal cells in vitro in order to provide the basic data for elucidating the role of AGM -derived-stromal cells in embryo-hematopoiesis and its hematopoietic suppoitive effect. RT-PCR was used to analyze the expression of IL-6, SCF, Flt3-L, oncostatin M (OSM), IL-3, TPO, M-CSF and LIF in human aorta-gonad-mesonephros-derived stromal cells (hAGMS1-S5) at mRNA level. IL-6, SCF and Flt3-L levels were detected in the supernatant of hAGMS1-S5 stromal cells by ELISA assay. Umbilical cord blood CD34(+) cells were cocultured with hAGMS1-S5 feeder cells, and hematopoietic cells were collected at day 14 for colony analysis in methylcellulose semisolid medium. The results showed that human aorta-gonad-mesonephros-derived stromal cells S1-S5 expressed IL-6, SCF, Flt3-L and OSM mRNA, but did not express IL-3, TPO, M-CSF and LIF mRNA. In the supernatant of hAGMS1-S5 cells, IL-6, SCF and Flt3-L could be detected by ELISA assay at different levels, while there was no significant difference between groups of hAGMS1-S5 (P > 0.05). When cocultured with umbilical cord blood CD34(+) cells, hAGMS1-S5 could support the expansion of CFU-GM, BFU-E, and CFU-Mix. The supportive effects of hAGM S1-S5 were significantly different (P < 0.05), hAGM S3 and S4 were better than hAGM S1, S2, and S5. It is concluded that detection of hematopoietic growth factors expressed in human aorta-gonad-mesonephros-derived stromal cells provided a solid foundation to elucidate the mechanism of hematopoiesis and the hematopoietic supportive effect of these stromal cells.
