Molecular basis of Rh DEL phenotype in Zhejiang Han population.
- Author:
An-Xin CHEN
1
;
Jun-Jie WU
;
Feng-Juan XU
;
Li-Ying ZHANG
;
Ying-Hua NI
;
Qi-Hua FU
Author Information
1. Department of Transfusion Medicine, Jinhua Central Hospital, Jinhua 321000, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
Base Sequence;
Blood Donors;
China;
ethnology;
Erythrocytes;
immunology;
Exons;
genetics;
Humans;
Molecular Sequence Data;
Phenotype;
Point Mutation;
Polymerase Chain Reaction;
methods;
Polymorphism, Genetic;
Rh-Hr Blood-Group System;
genetics;
immunology;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2006;14(5):1029-1032
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the molecular basis of Rh DEL phenotype. Rh DEL phenotypes were identified by a serologic adsorption-elution method, the nucleotide sequences of ten RHD exons and exon-intron boundary regions were evaluated by a RHD gene-specific PCR-SSP (PCR-SSP, polymerase chain reaction-sequence specific primer) and sequencing. The results showed that out of 122 random Rh negative donors 35 Rh DEL phenotypes were identified through serologic method, including 6 RhCCdee (17.14%), 28 RhCcdee (80.00%), and 1RhCcdEe (2.86%). Sequence analysis indicated that all DEL phenotypes harbored a RHD 1227 G > A mutation in exon 9. D zygosity test revealed that 29 DEL phenotypes (28 RhCcdee and 1 RhCcdEe) had one RHD gene deleted, and 6 DEL phenotypes (6 RhCCdee) had homogenous RHD gene. It is concluded that RHD 1227A is an important genetic marker for Rh DEL phenotype in Zhejiang Han population.
