Production of specific CTL induced by exosomes derived from K562 cells.
- Author:
Shao-Qian CHEN
1
;
Ying DU
;
Xin WANG
;
Qiao-Li GU
;
Yu-Min HUANG
;
Zi-Ming DONG
Author Information
1. Department Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
- Publication Type:Journal Article
- MeSH:
Dendritic Cells;
cytology;
immunology;
Endosomes;
immunology;
Exocytosis;
immunology;
Humans;
K562 Cells;
Monocytes;
cytology;
RNA, Neoplasm;
genetics;
immunology;
T-Lymphocytes, Cytotoxic;
immunology;
Transfection
- From:
Journal of Experimental Hematology
2006;14(6):1168-1171
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate whether exosomes derived from K562 cells and human monocyte-derived dendritic cells (DCs) transfected with total RNA of K562 cells are capable of inducing antigen-specific cytotoxic T lymphocytes (CTL) responses in vitro. DCs were generated from peripheral blood mononuclear cells (PBMNC) of healthy volunteers in the presence of GM-CSF and IL-4, and then were transfected with K562 RNA by using DOTAP lipofection. Exosomes was extracted from the supernatant of DCs and K562 cells. The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers' PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from K562 cells or DCs transfected with total RNA of K562 cells could significantly promote their killing ability on K562 cells as compared with untreated T cells (P < 0.05). The killing ability of T cells treated with exosomes on K562 cells was stronger than on HL-60 cells (P < 0.05). It is concluded that the specific CTL immune response to leukemia cells can be induced by exosomes derived from K562 cells.
