Feasibility of HLA-DRB1 matching by using DHPLC.
- Author:
Jing-Bo WANG
1
;
Dan LI
;
Kai-Feng PAN
;
Dao-Pei LU
Author Information
1. Institute of Hematology, Peking University People Hospital, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Blood Donors;
Chromatography, High Pressure Liquid;
methods;
Feasibility Studies;
HLA-DR Antigens;
genetics;
immunology;
HLA-DRB1 Chains;
Hematopoietic Stem Cell Transplantation;
Histocompatibility Testing;
methods;
Humans;
Molecular Sequence Data;
Polymorphism, Genetic;
genetics
- From:
Journal of Experimental Hematology
2006;14(6):1183-1187
- CountryChina
- Language:Chinese
-
Abstract:
To study feasibility of HLA-DRB1 matching by using denatured high performance liquid chromatography (DHPLC), 20 pairs of DNA samples from donors and recipients of hematopoietic cell transplantation (HCT) for DRB1 matching and 2 pairs of samples from donors and recipient of HCT for DRB1 mismatching were studied by DHPLC and PCR-SSP. After being amplified and annealed slowly to produce heteroduplex, PCR products for exon 2 of DRB1 were detected by DHPLC to find matched or mismatched peaks in chromatogram. The results showed that DHPLC and PCR-SSP were consistant with matched or mismatched HLA-DRB1 typing. The results of DHPLC and PCR-SSP for matching were compared by using kappa test (kappa = 0.776, P = 0.00), which suggested DHPLC for HLA-DRB1 matching was in agreement with PCR-SSP. In conclusion, DHPLC for HLA-DRB1 matching is economic and convenient, moreover, will not be affected by unknown genes in HLA-DRB1 locus.
