Sequence analysis of HLA-B*4061 allele newly found.
- Author:
Wei ZHANG
1
;
Qin-Feng LÜ
;
Wei WANG
;
Zhe-Dong HAN
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Institute of Immunology, Medical College of Zhejiang University, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Amino Acid Substitution;
Asian Continental Ancestry Group;
genetics;
Base Sequence;
China;
HLA-B Antigens;
genetics;
immunology;
Histocompatibility Testing;
Humans;
Molecular Sequence Data;
Sequence Analysis, DNA;
Sequence Homology, Nucleic Acid
- From:
Journal of Experimental Hematology
2006;14(6):1188-1190
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was aimed to investigate the molecular genetic basis for a novel HLA allele, HLA-B*4061, in Chinese population. DNA was extracted from whole blood by salting-out method. The HLA-B exons 1 - 8 of the proband was amplified and the amplified product was cloned using TOPO TA cloning sequencing kit to split the two alleles apart. Both strands of exons 2, 3 and 4 of chosen colonies were sequencing. The PCR-SSP was performed to confirm the mutations detected by sequencing. The sequencing results showed HLA-B alleles of the proband as B*4601 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ089628, DQ089629, DQ089630). After HLA blast analysis, the novel allele showed a single nucleotide mismatch with B*400101 in exon 2 at position 272 C-->A, as the results, changing amino acid from Ser to Tyr at codon 67. It is concluded that this allele is a novel one and has been officially named B*4061 by the WHO Nomenclature Committee.
