Role of bone marrow-derived mesenchymal stem cells in reduction of graft-versus-host disease by effecting CD4+CD25+ regulatory T cells in rats.
- Author:
Ying TIAN
1
;
Yu-Bin DENG
;
Yi-Jun HUANG
;
Xiao-Dong NA
;
Yan LI
;
Mei-Hong YE
Author Information
1. Department of Pathophysiology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Bone Marrow Transplantation;
adverse effects;
CD4-Positive T-Lymphocytes;
immunology;
CD8-Positive T-Lymphocytes;
immunology;
Graft vs Host Disease;
immunology;
Interleukin-2 Receptor alpha Subunit;
immunology;
Mesenchymal Stromal Cells;
immunology;
physiology;
Rats;
Rats, Inbred F344;
Rats, Wistar;
T-Lymphocytes, Regulatory;
immunology
- From:
Journal of Experimental Hematology
2006;14(6):1210-1214
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to investigate the effects and mechanism of bone marrow-derived mesenchymal stem cells (MSCs) on graft-versus-host desease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The model of GVHD in rat had been established by allo-HSCT with donor derived T cells. The occurence of GVHD in recipients was observed in condition with or without donor derived MSC co-transplantation. Effects of MSCs on GVHD were analyzed by model rat survival rate and pathology. Proportions of CD4+CD25+ regulatory T cells were determined by using label spleen lymphocytes and thymocytes with double fluorescent-labeled antibodies and flow cytometry. The results showed that MSCs inhibited the lethal GVHD after HSC co-transplantation and increased the survival rate. The ratio of CD4/CD8 deceased in GVHD group in different levels, as compared with that in the experimental group. The proportion of CD4+CD25+ regulatory T cells of spleen lymphocytes was 31.55 +/- 7.58% and 20.90 +/- 1.90% in experimental and GVHD groups, respectively. Similarly, the proportion of CD4+CD25+ regulatory T cells of thymocytes was 93.20 +/- 2.69% and 57.17 +/- 6.79% in experimental and the GVHD groups, respectively. Meanwhile the proportion of CD4+CD25+ regulatory T cells was higher in experimental group than that in GVHD group. It is concluded that MSCs may prevent the lethal GVHD after allo-HSC co-transplantation and raise the survival rate of model rats by acting on the CD4+CD25+ regulatory T cells in vivo.
