PPARalpha agonist--fenofibrate inhibits LPS-induced tissue factor expression in THP-1 cells.
- Author:
Chun-Xia DONG
1
;
Yu HU
;
Hua-Fang WANG
;
Chun-Yan SUN
;
Ya-Dan WANG
;
Wen-Juan HE
;
Xiao-Ping ZHANG
Author Information
1. Institut of Hematology, Union Hospital, Tongji Medical College, Huazhong Univesity of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Depression, Chemical;
Fenofibrate;
pharmacology;
Humans;
Leukemia, Monocytic, Acute;
metabolism;
pathology;
Lipopolysaccharides;
antagonists & inhibitors;
pharmacology;
PPAR alpha;
agonists;
RNA, Messenger;
biosynthesis;
genetics;
Thromboplastin;
biosynthesis;
genetics;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2006;14(6):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the influence of PPARalpha agonist on the expression of TF (tissue factor) in THP-1 cells. THP-1 cells were pretreated with different concentrations of PPARalpha agonist (fenofibrate) for definite time. Lipopolysaccharide (LPS)-induced TF mRNA and protein levels were detected by RT-PCR and Western blot respectively. The results showed that fenofibrate decreased tissue factor protein and mRNA expression in supernatants of LPS-stimulated human monocytes in a concentration-dependent manner (P < 0.05 - 0.01, n = 5). It is concluded that fenofibrate inhibit TF expression induced by LPS in THP-1 cells, which may be involved in the anti-atherosclerotic effects of PPARalpha agonist.
