Signaling pathways regulating self-renewal of mouse embryonic stem cells--review.
- Author:
Xiao-Yan WANG
1
;
Bing LIU
;
Ning MAO
Author Information
1. Institute of Basic Medical Science, Acadecy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
physiology;
Cell Proliferation;
Cell Survival;
Cells, Cultured;
Cytokine Receptor gp130;
metabolism;
Embryonic Stem Cells;
cytology;
physiology;
Janus Kinase 1;
metabolism;
Leukemia Inhibitory Factor;
metabolism;
Mice;
STAT3 Transcription Factor;
metabolism;
Signal Transduction;
physiology
- From:
Journal of Experimental Hematology
2006;14(6):1248-1252
- CountryChina
- Language:Chinese
-
Abstract:
Mouse embryonic stem cells (ES cells) are pluripotent in that they can give rise to almost all the cell types in vitro and in vivo. Also, they can sustain self-renewal in vitro owing to symmetrical mitosis, i.e., only the cell number increases while the daughter cells remain pluripotent. Self-renewal and pluripotency of ES cells are under stringent regulation of several signaling pathways. Activation of either JAK-STAT3 or PI3K, the downstream cascade of gp130, can maintain the self-renewal of ES cells, while phosphorylation of another gp130-related branch, SHP2-Ras-ERK, drives the differentiation. BMP2/4-mediated signaling is capable of suppressing the differentiation of ES cells in collaboration with activated JAK-STAT3 under serum free culture conditions. Other signaling such as Wnt also contributes to the self-renewal of ES cells. Generally, the network, which is composed of various signaling pathways, modulates the self-renewal and differentiation of mouse ES cells precisely. This review focuses on the role of gp130 in proliferation of mouse ES cells including inhibitory effect of JAK-STAT3 pathway activation on differentiation of mouse ES cells, maintenance effect of PI3K pathway activation on self-renewal of ES cells, promotive effect of SHP-2-Ras-ERK pathway activation on differentiation of ES cells, and influence of other signaling pathways on self-renewal of mouse ES cells, including maintenance effect of BMP combination with LIF under serum free culture conditions on self-renewal of ES cells and promotive effect of Wnt pathway activation on self-renewal of ES cells.
