LRP15 gene promoter region methylation and its expression in acute leukemia.
- Author:
Yu ZHAO
1
;
Li-Ping DOU
;
Quan-Shun WANG
;
Xue-Chun LU
;
Fang-Ding LOU
;
Li YU
Author Information
1. Department of Hematology, The General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
DNA Methylation;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Leukemia, Myeloid, Acute;
genetics;
pathology;
Male;
Neoplasm Proteins;
biosynthesis;
genetics;
metabolism;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
pathology;
Promoter Regions, Genetic;
genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2007;15(1):39-41
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the relationship between LRP15 gene promoter region methylation and its gene expression in acute leukemia patients, the status of LRP15 gene promoter region methylation was detected by MS-PCR and the gene expression was detected by RT-PCR in bone marrow samples from leukemia patients. The results indicated that the LRP15 gene expression was 47.6% in complete remission (CR) patients and 16.7% in non-CR patients respectively, while LRP15 gene promoter region methylation was 38.1% in CR group and 72.2% in non-CR group respectively. No relationship was found between LRP15 gene promoter region methylation and its expression (P = 0.0087). It is concluded that the methylation in LRP15 gene promoter region may not be the only reason for LRP15 gene silence.
