Effects of different oxygen flow rates on myocardial ischemia-reperfusion injury in rabbits.
- Author:
Wei GONG
1
;
Jian-Xin LIU
;
Long-Yu JIN
;
Dian-Jun WANG
;
Chao HONG
;
Zhi-Bin JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cardiopulmonary Bypass; Myocardial Reperfusion Injury; prevention & control; Oxygen; administration & dosage; Rabbits; Reperfusion; methods
- From: Journal of Southern Medical University 2009;29(7):1477-1478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of different oxygen flow rates during cardiopulmonary bypass (CPB) on myocardial ischemia-reperfusion (IR) injury in rabbits.
METHODSThirty rabbits were randomized equally into groups A, B and C to receive controlled oxygen reperfusion at low, normal and high flow rates (25, 50, and 80 ml.kg(-1).min(-1), respectively). Serum concentration of CK-MB and cTnT were tested by ELISA before the operation (T0) and after 30 min (T1), 2 h (T2), 12 h (T3) and 24 h (T4) of reperfusion. W/D, SOD and MDA of the myocardium were determined before and at 60 min after reperfusion. The ultrastructural alterations of the myocardium were observed.
RESULTSSerum concentration of CK-MB and cTnT in the 3 groups increased significantly after the operation, and their levels were the lowest in group A (P<0.05). W/D and MDA in the myocardium was also the lowest, while SOD the highest in group A (P<0.05). Ultrastructural pathologies were found in all the 3 groups, but relatively mild in group A.
CONCLUSIONLow oxygen flow rate during controlled reperfusion may protect the myocardium from IR injury in rabbits.
