Pharmacokinetic study of polydatin in Beagle dogs.
- Author:
Zhuo CAI
1
;
Fa YU
;
An-de MA
;
Xue-mei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; Dogs; Female; Glucosides; blood; pharmacokinetics; Limit of Detection; Linear Models; Male; Reproducibility of Results; Stilbenes; blood; pharmacokinetics
- From: Journal of Southern Medical University 2009;29(8):1538-1540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for detecting plasma polydatin using high-performance liquid chromatography (HPLC) and investigate the pharmacokinetics of polydatin in Beagle dogs.
METHODSHPLC-based detection of polydatin was performed on a Hypersil-BDS C18 column with a mobile phase of methanol and water (35:65) at the flow rate of 1.0 ml/min and the detection wavelength of 290 nm. The pharmacokinetic parameters of polydatin were calculated by non-compartment model statistics.
RESULTSThe standard curve was linear within the range of 0.21-21.0 microg/ml (correlation coefficient of 0.9995, n=5). The average recovery was 90% with a relative standard deviation no more than 8.0%. The limit of detection of the method was 0.1 microg/ml. The pharmacokinetic parameters of polydatin at 3 doses were obtained, with T((1/2)); of 89.95-/+19.96 min, 152.15-/+55.82 min, and 202.36-/+66.75 min, and AUC0-infinity of 300.14-/+51.33 g.min.ml(-1), 751.72-/+173.97 g.min.ml(-1) and 1521.12-/+310.02 microg.min.ml(-1), respectively.
CONCLUSIONThe method we established allows effective detection of polydatin, whose pharmacokinetics conforms to the two-compartment model.
