Effect of protein kinase CK2 gene silencing on radiosensitization in human nasopharyngeal carcinoma cells.
- Author:
Li LIU
1
;
Jin-jin ZOU
;
He-san LUO
;
De-hua WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Annexin A5; metabolism; Casein Kinase II; deficiency; genetics; metabolism; Cell Line, Tumor; Histones; genetics; Humans; Nasopharyngeal Neoplasms; genetics; pathology; RNA Interference; RNA, Small Interfering; genetics; Radiation Tolerance; genetics; Transfection
- From: Journal of Southern Medical University 2009;29(8):1551-1553
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of protein kinase CK2 gene silencing on the radiosensitization in human nasopharyngeal carcinoma (NPC) cells and its possible mechanism.
METHODSRNA interference (RNAi) technique was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells, and clonogenic assay was employed to observe the changes in the radiosensitivity of the cells. DNA double-strand break was assessed by immunofluorescence staining of gamma-H2AX foci, and the cell apoptosis was examined using Annexin V-FITC/PI double-staining flow cytometry.
RESULTSCK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the clonogenic activity and increased the radiosensitivity of the NPC cells. After a 15-min exposure of the cells to 1 Gy radiation, significant difference occurred in the gamma-H2AX foci between CK2alpha knockdown cells and the control cells (P<0.01). CK2alpha silencing significantly increased the cell apoptosis after the exposure (P<0.01).
CONCLUSIONSProtein kinase CK2 plays an important role in the radiosensitivity of the NPC cells, and suppression of its expression might be a potential therapeutic approach of cancer.
