Effects of epigallocatechin-3-gallate on the proliferation of colonic cancer cell line SW620 and PAK1 gene expression.
- Author:
Xue-qing CHEN
1
;
Jin-bao WU
;
Xiao-qiang YANG
;
Yu-gang SONG
;
Ming-song LI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Catechin; analogs & derivatives; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Colonic Neoplasms; genetics; pathology; Gene Expression Regulation, Neoplastic; drug effects; Humans; p21-Activated Kinases; metabolism
- From: Journal of Southern Medical University 2009;29(8):1568-1570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of epigallocatechin-3-gallate (EGCG) on the proliferation of SW620 cells and the expression of PAK1 gene.
METHODSHuman colonic cancer cell line SW620 was treated with EGCG at 40, 60 and 80 micromol/L and cultured in RPMI 1640 medium for 0, 24, 48 and 72 h. The proliferation of SW620 cells was observed by MTT assay before and after EGCG treatment, and the expression of PAK1 protein was observed by Western blotting.
RESULTSSW620 cells treated with EGCG displayed a slowed growth in comparison with the control cells, and the growth rate decreased with the increase of EGCG concentration. PAK1 protein expression was lowered in SW620 cells after EGCG treatment for 48 h.
CONCLUSIONEGCG can inhibit the proliferation and partially reduce the expression of PAK1 protein in SW620 cells.
