Identification of human testicular embryonal carcinoma proteins by two-dimensional gel electrophoresis and mass spectrometry.
- Author:
Fei LI
1
;
Ya-guang ZOU
;
Qi-zhao ZHOU
;
Tie-qiu LI
;
Wen-bin GUO
;
Xiao-wei JING
;
Xiang-ming MAO
;
Wan-long TAN
;
Shao-bin ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biomarkers, Tumor; analysis; metabolism; Carcinoma, Embryonal; genetics; metabolism; pathology; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Neoplastic; Humans; Male; Mass Spectrometry; Proteomics; methods; Testicular Neoplasms; genetics; metabolism; pathology; Young Adult
- From: Journal of Southern Medical University 2009;29(8):1585-1587
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry.
METHODSImmobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS).
RESULTS2-DE effectively screened the differentially expressed proteins in the carcinoma tissues. Three proteins highly expressed in the carcinoma were successfully identified.
CONCLUSIONThe proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.
