An improved liquid nitrogen freezing method for establishing canine models of femoral head necrosis.
- Author:
Guo-zhu LIU
1
;
Tong-tao YANG
;
Jian-wei HAN
;
Jin WU
;
Yan-hua WEN
;
Jie GAO
;
Bao-an MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Dogs; Female; Femur Head Necrosis; Freezing; Male; Nitrogen; chemistry
- From: Journal of Southern Medical University 2009;29(8):1602-1604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method.
METHODSSixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head.
RESULTSSignificant radiographic, gross and pathological changes occurred in the femoral head after the freezing.
CONCLUSIONImproved liquid nitrogen freezing of the femoral head provides a simple and convenient method for establishing animal models of femoral head osteonecrosis.
