Changes of endothelial cell function and platelet activation in rabbit spinal cord with ischemia-reperfusion injury.
- Author:
Shou-ping GONG
1
;
Wen-tao WANG
;
Da-lin ZHONG
;
Jian LV
;
Feng WU
;
Jin CHE
;
Zhi-yuan SENG
;
Xi-jing HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Endothelial Cells; metabolism; Nitric Oxide; blood; P-Selectin; blood; Platelet Activation; Rabbits; Reperfusion Injury; blood; pathology; physiopathology; Spinal Cord; pathology; physiopathology
- From: Journal of Southern Medical University 2009;29(8):1638-1640
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the changes of vascular endothelial cell function and platelet activation in rabbit spinal cord following ischemia-reperfusion (I/R) injury and their roles in the spinal cord injury.
METHODSRabbit spinal cord I/R injury models were established using Zivin method, and the changes in plasma NO and GMP140 levels were dynamically monitored after the injury.
RESULTSPlasma NO level increased significantly in the I/R group at the end of the ischemia, and reached the peak level at 2 h of reperfusion as compared to that in sham-operated group (P<0.01). Plasma NO level decreased at 6 h of reperfusion, but still significantly higher than the level in the sham-operated group (P<0.05). Plasma GMP140 underwent no significant changes in the sham-operated group, but significantly increased in the I/R group at the end of the ischemia, followed by gradual declination to the normal level at 2 h of reperfusion.
CONCLUSIONSpinal cord I/R injury causes overexpressions of NO and GMP140, suggesting the involvement of endothelial cell injury and platelet overactivation in the pathological process and repair of spinal cord I/R injury.
