Effects of peritoneal dialysis solution on apoptosis and intracellular free calcium, cell surface ICAM-1 expression of rat peritoneal mesothelial cells.
- Author:
Hao REN
1
;
Sheng-xiao CHEN
;
Hong-fa LIU
;
Zheng-rong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Calcium; metabolism; Dialysis Solutions; pharmacology; Dose-Response Relationship, Drug; Epithelial Cells; cytology; drug effects; metabolism; Gene Expression Regulation; drug effects; Glucose; pharmacology; Intercellular Adhesion Molecule-1; metabolism; Intracellular Space; drug effects; metabolism; Male; Peritoneal Cavity; cytology; Peritoneal Dialysis; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2009;29(8):1752-1754
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of peritoneal dialysis solution (PDS) on apoptosis and intracellular free calcium([Ca(2+)]i), cell surface ICAM-1 expression of rat peritoneal mesothelial cells (RPMCs).
METHODSThe RPMCs apoptosis rate were determined by flow cytometry. [Ca(2+)]i in the cells were monitered the fluorescence at 528 nm by confocus laser microscopy. Cell surface ICAM-1 expression were detected by flow cytometry.
RESULTAfter PDS treatment for 1 h, the RPMCs apoptosis rate were increased. Such increase was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. At the same times, after 3 hours, ICAM-1 expressions of the PDS containing glucose and mannitol are all increased. With the increase of glucose concentrations, the descend of [Ca(2+)]i levels were aggravated.
CONCLUSIONPDS containing high- concentration glucose can induce significant apoptosis of RPMCs in vitro. This may be related with the enhanced level of ICAM-1 expressions and the decreased level of [Ca(2+)]i. Which may due to the occurrence of peritoneal fibrosis and ultrafiltrate failure in patients suffering long term peritoneal dialysis.