Effects of apoptotic lymphocytes on proinflammatory cytokine secretion by hepatic sinusoidal endothelial cells.
- Author:
Dong HE
1
;
Yu-Wei LUO
;
Li MA
;
Er-Wei SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Cells, Cultured; Coculture Techniques; Cytokines; secretion; Down-Regulation; Endothelial Cells; cytology; metabolism; Humans; Immune Tolerance; Interferon-gamma; secretion; Interleukin-2; secretion; Liver; cytology; Lymphocytes; cytology; Tumor Necrosis Factor-alpha; secretion
- From: Journal of Southern Medical University 2009;29(6):1127-1129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of apoptotic lymphocytes on the secretion of cytokines by hepatic sinusoidal endothelial cells (HSEC).
METHODSHuman HSEC cells were co-cultured for 16 h with allogenetic apoptotic lymphocytes induced by UVB irradiation. The supernatants were collected and the levels of interleukin-2, interferon-gamma, and tumor necrosis factor-alpha were detected by Luminex technique.
RESULTSAll the cytokines were down-regulated by about 50% in HSECs after co-culture with the apoptotic lymphocytes as compared with those in the control group (P<0.05).
CONCLUSIONSCo-culture with apoptotic lymphocytes can down-regulate the secretion of pro-inflammatory cytokines in HSECs, which may contribute to tolerogenic microenvironment in the liver.
