Effects of Astragalus Membranaceus Injection on TNF-alpha-induced release of inflammatory factors from HUVECs and the molecular mechanisms.
- Author:
Ze-Bin YE
1
;
Fan-Jun WANG
;
Gang-Qing ZHANG
;
Wang-Min YANG
;
Hai-Sen QIN
;
Xing-Tao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus membranaceus; chemistry; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; cytology; metabolism; Humans; Intercellular Adhesion Molecule-1; metabolism; Interleukin-6; metabolism; Transcription Factor RelA; metabolism; Tumor Necrosis Factor-alpha; antagonists & inhibitors; pharmacology; Umbilical Veins; cytology
- From: Journal of Southern Medical University 2009;29(6):1137-1140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of Astragalus Membranaceus Injection on human umbilical vein endothelial cells (HUVECs) against tumor necrosis factor alpha (TNF-alpha).
METHODSCultured passage 2 HUVECs were stimulated with TNF-alpha with or without a 2-h Astragalus Membranaceus Injection treatment. The expression of nuclear factor-kappaB (NF-kappaB) subunit p65 were evaluated by immuncytochemistical method, and the levels of p65 in the nuclei and the protein Ikappabetaalpha in the cytoplasm were evaluated by Western blotting. The levels of interleukin-6 (IL-6) and soluble intracellular adhesion molecule-1 (sICAM-1) in the cell culture were determined with ELISA.
RESULTSTNF-alpha induced the activation of NF-kappaB and increased the expressions of IL-6 and sICAM-1 in HUVECs. The activation of NF-kappabeta by TNF-alpha was suppressed by Astragalus Membranaceus Injection in a dose-dependent manner.
CONCLUSIONAstragalus Membranaceus Injection can inhibit the TNF-alpha-induced expression of IL-6 and sICAM-1 by suppressing NF-kappabeta activation, suggesting its protective effect on the endothelial function.
