Effect of RNA interference targeting-survivin on the invasiveness of human glioma cells in vitro.
- Author:
De-Gang SHI
1
;
Yu FAN
;
Fu ZHU
;
Ji-Ren ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Brain Neoplasms; genetics; pathology; Cell Line, Tumor; Glioma; genetics; pathology; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; metabolism; Neoplasm Invasiveness; genetics; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Urokinase-Type Plasminogen Activator; genetics; metabolism
- From: Journal of Southern Medical University 2009;29(6):1156-1158
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of survivin gene in the invasive behavior of glioma cells and explore the possible mechanism.
METHODSThe mRNA and protein expressions of survivin in glioma cell line SNB19 transfected by small interfering RNA (siRNA) targeting survivin were determined by real time RT-PCR and Western blotting, respectively. The anchorage-independent growth of the cells was examined by clone formation assay in soft agar, and their invasiveness was evaluated using a Boyden chamber model. The protein level of urokinase-type plasminogen activator (uPA) was also determined by western blotting.
RESULTSSurvivin siRNA dose-dependently inhibited the anchorage-independent growth and invasiveness and reduced the expression of uPA protein in SNB19 cells.
CONCLUSIONRNA interference targeting survivin can inhibit the invasiveness of glioma cells in vitro possibly by down-regulating uPA expression.
