Antisense oligonucleotides targeting protein kinase C alpha inhibits the proliferation of A549 cells.
- Author:
Yu-Xia YAN
1
;
Jian-Wei JIANG
;
Zhi-Hong HUANG
;
Zhi-Hui WU
;
Chun-Lan LIN
;
Feng-Yun WU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; drug effects; Humans; Lung Neoplasms; enzymology; pathology; Oligonucleotides, Antisense; genetics; pharmacology; Protein Kinase C-alpha; genetics; metabolism; RNA, Messenger; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2009;29(6):1189-1191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of antisense oligonucleotides (ASODN) targeting protein kinase C alpha (PKCalpha) on the proliferation of A549 cells.
METHODSPKCalpha ASODN and random oligonucleotides (RODN) were transfected into A549 cells mediated by polyethyleneimine, and the proliferation and clone formation of A549 cells were detected by CCK-8 and clone formation assay, respectively. The expression of PKCalpha in the transfected cells was analyzed by RT-PCR and Western blotting.
RESULTSCompared with those in the control group, PEI group and PEI-RODN group, the proliferation and clone formation of A549 cells treated with ASODN targeting PKCalpha were significantly inhibited (P<0.05). The expressions of PKCalpha mRNA and protein in PKCalpha ASODN-transfected A549 cells were significantly lower than those in the other 3 groups (P<0.05).
CONCLUSIONThe PKCalpha ASODN mediated by PEI down-regutates the expression of PKCalpha gene and suppress the proliferation and clone formation of A549 cells.
