Effects of mitogen-activated protein kinases signaling pathway proteins on kidney injury in mice exposed subchronically to cadmium.
- VernacularTitle:亚慢性镉暴露BALB/c小鼠丝裂原活化蛋白激酶信号通路蛋白在肾损伤中的作用
- Author:
Jin-Xia ZHAI
1
;
Zhao-Xiang ZHANG
;
Shu-Shu DING
;
Ya-Juan FENG
;
Xing-Hua WANG
;
Rui DING
;
Dao-Jun CHEN
;
Qiu-Yue YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cadmium; toxicity; Extracellular Signal-Regulated MAP Kinases; metabolism; Female; JNK Mitogen-Activated Protein Kinases; metabolism; Kidney; metabolism; pathology; MAP Kinase Signaling System; Mice; Mice, Inbred BALB C; Mitogen-Activated Protein Kinases; metabolism; Signal Transduction; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(9):670-673
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of mitogen activated protein kinase (MAPK) and extracellular signal-regulated kinases (ERK) on kidney injury in female BALB/c mice exposed to cadmium.
METHODTwenty-one female BALB/c mice were randomly divided into 3 groups, i.e. control group, low Cd exposure group (2.5 µmol/kg) and high Cd exposure group (10 µmol/kg) were exposed to normal saline, 2.5, 10 µmol/kg Cd, respectively, 3 times a week for 14 weeks. The kidney slice were stained by HE, PAS and Masson staining to observe the morphological changes. The expression levels of pERK, ERK, pp38, p38, pJNK and JNK proteins in kidneys were tested by Western blot assay.
RESULTSThe ratios of pERK/ERK, pp38/p38, pJNK/JNK in high Cd group were higher than those in the control group (P < 0.05). The ratio of pERK/ERK in low Cd group was higher than control group (P < 0.05). The expression levels of bcl-2, bax proteins and the ratio of bcl-2 to bax in Cd exposure groups decreased significantly, as compared with the control group (P < 0.05). The impairment of renal glomeruli and tubules were observed in HE, PAS and Masson staining slices of kidneys in mice exposed to Cd.
CONCLUSIONCdCl2 may induced renal injury by affecting the expression levels of MAPK.
