The toxic effects of lead acetate on the apoptosis and the ultrastructure in human renal tubular epithelial cells (HK-2).
- Author:
Qing-Hua JIA
1
;
Xiao-Qin HA
;
Xiao-Peng YANG
;
Ye-Wei CHANG
;
Zhi-Hua YANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line; Epithelial Cells; cytology; drug effects; ultrastructure; Humans; Kidney Tubules, Proximal; cytology; drug effects; ultrastructure; Organometallic Compounds; toxicity
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(9):674-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the toxic effects of lead acetate on the apoptosis and ultrastructure of human renal tubular epithelial cells (HK-2).
METHODSAfter HK-2 cells were exposed to 5, 10 and 20 µmol/L lead acetate for 24 h, the morphological changes of HK-2 cells were observed by Hochest 33342-PI staining, and the ultrastructure changes of HK-2 cells were examined under a electron microscope, LDH activity and MDA content in supernatant of HK-2 cellular culture were detected by spectrophotometer, DNA damage of HK-2 was determined by DNA ladder and the apoptotic rates of HK-2 cells were measured by flow cytometry.
RESULTSThe morphological changes of apoptotic HK-2 cells in exposure group were observed by Hochest 33342-PI staining. The cytoplasm vacuoles, karyopycnosis, nuclear membrane vague and apoptotic bodies in HK-2 cells of exposure group were found under electron microscopy. LDH activity and MDA contents in exposure group increased significantly, as compared to control group (P < 0.01). The results of DNA Ladder showed that DNA damage of HK-2 cells in exposure group appeared. The apoptotic rates of HK-2 cells exposed to 5, 10, 20 µmol/L lead acetate were 14.16% ± 2.94%, 19.45% ± 2.73%, 25.01% ± 3.97%, respectively, which were significantly higher than that (5.81% ± 2.18%) in control group (P < 0.05).
CONCLUSIONLead acetate could remarkably induce the apoptosis of HK-2 cells and affect the kidney.