The change of telomere protein in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts.
- VernacularTitle:煤焦沥青烟提取物致BEAS-2B恶性转化细胞端粒蛋白的变化
- Author:
Wei WANG
1
;
Zhi-Tao LI
;
Han-Song ZHU
;
Yong ZHAO
;
Li-Xia WANG
;
Zhen YAN
;
I Shi-En L
;
Dong XU
;
Wei-Dong WU
;
Yong-Jun WU
;
Yi-Ming WU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Transformation, Neoplastic; metabolism; Coal Tar; toxicity; Epithelial Cells; cytology; metabolism; pathology; Humans; Repetitive Sequences, Nucleic Acid; Telomere-Binding Proteins; genetics; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(9):678-681
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBy testing the changes of telomere binding protein in malignant transformation BEAS-2B cells induced by coal tar pitch smoke extracts, to study the role of protection of telomeres 1 (POT1), telomeric repeat binding factor 1 (TRF1) and TRF2 in tumorgenesis that contact with coal tar pitch.
METHODSThe BEAS-2B cells were induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, the protein expression variations were determined by cell culture overslip of immunohistochemical methods.
RESULTSIn malignant transformation cells, the mRNA expression level (POT1: 0.63 ± 0.04, TRF1: 0.36 ± 0.01) and the protein expression level (POT1: 0.36 ± 0.05, TRF1: 0.09 ± 0.03) of POT1 and TRF1 was statistically significant decreased compared to that of BEAS-2B group (mRNA: POT1: 1.00 ± 0.04, TRF1: 1.01 ± 0.16; protein: POT1: 0.55 ± 0.07, TRF1: 0.27 ± 0.07) and DMSO group (mRNA: POT1: 0.89 ± 0.12, TRF1: 0.90 ± 0.08; protein: POT1: 0.55 ± 0.10, TRF1: 0.26 ± 0.04) (P < 0.05); mRNA expression level (1.45 ± 0.07) and the protein expression level (0.88 ± 0.06) of TRF2 was increased compared to that of BEAS-2B group (mRNA: 1.00 ± 0.07, protein: 0.48 ± 0.06) and DMSO group (mRNA: 1.00 ± 0.06, protein: 0.50 ± 0.06) (P < 0.05).
CONCLUSIONThe change of gene and protein expression level in POT1, TRF1, and TRF2 involved in the process that evolved into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.
