Two-dimensional electrophoresis and mass spectrometry analysis on effects of trichloroethylene on protein of L-02 liver cells.
- Author:
Hai-yan HUANG
1
;
Jian-jun LIU
;
Zhi-xiong ZHUANG
;
Xi-yi LI
;
Xin-yun XU
;
Qin-zhi WEI
;
Xiao-hua YANG
Author Information
- Publication Type:Journal Article
- MeSH: Anesthetics, Inhalation; pharmacology; Cell Line; Electrophoresis, Gel, Two-Dimensional; methods; Hepatocytes; cytology; drug effects; metabolism; Humans; Peptide Mapping; Proteins; analysis; Proteome; analysis; Proteomics; methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods; Trichloroethylene; pharmacology
- From: Chinese Journal of Preventive Medicine 2005;39(3):175-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of trichloroethylene (TCE) on the protein in L-02 cells in vitro.
METHODSThiazolyl blue and Trypan blue tests were used to investigate the cytotoxicity of TCE to L-02 liver cell. The 2-D electrophoresis was used to analyse the expression of proteins in L-02 liver cells. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).
RESULTSWhen the concentration of TCE exceeded 30 micromol/L, there was distinct cytotoxicity to L-02 cell (P < 0.05). Selected 40 micromol/L to treat L-02 liver cells and analyze the differential proteome expression, the results showed that the expression level of 37 protein spots was up-regulated and 15 protein spots was down-regulated. And 15 proteins were identified by MALDI-TOF-TOF-MS.
CONCLUSIONTCE can change the proteome expression of L-02 liver cell. It should provide the fundamental information to identify proteins related to TCE in further study.
