Gene expression of resistin and the effect of conjugated linoleic acid on its expression of obese rat fed with high fat diet.
- Author:
Xiao-rong ZHOU
1
;
Chang-hao SUN
;
Xiao-hong ZHANG
;
Hai-ying WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; drug effects; metabolism; Animals; Dietary Fats; administration & dosage; Gene Expression; drug effects; Insulin Resistance; Linoleic Acids, Conjugated; pharmacology; Male; Obesity; etiology; genetics; PPAR gamma; genetics; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Wistar; Resistin; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Preventive Medicine 2005;39(3):191-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the gene expression of the resistin and the effects of conjugated linoleic acid on its expression in white adipose tissue of obese rats fed with high fat diet during the formation of insulin resistance.
METHODSMale Wistar rats were randomly separated in control group, high-fat group and high fat + conjugated linoleic acid (CLA) group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight), using reverse transcription polymerase chain reaction (RT-PCR) technique to measure the expression level of resistin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA expression.
RESULTSthe serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) mIU/L, (5.09 +/- 0.66) mmol/L, and supplement of CLA might decrease hyperinsulinemia and hyperglycemia, in CLA group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight) the serum insulin levels were (6.99 +/- 1.77) mIU/L, (7.36 +/- 1.48) mIU/L, (7.85 +/- 1.60) mIU/L, and glucose levels were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L, (4.06 +/- 0.63) mmol/L. The expression of resistin in adipose tissue of obese rat fed with high fat diet was increased as compared with those fed with basic diet. CLA might increase the expression of resistin and PPARgamma in adipose tissue of obese rat.
CONCLUSIONThe expression of resistin mRNA of obese rat fed with high fat diet was higher than those fed with basic diet, and CLA might improve the insulin resistance in obese rats and possibly upregulate the expression of resistin through activing PPARgamma.
