Effects of conjugated linoleic acid on the expression of critical enzymes of linoleic acid metabolism in tumor cell.
- Author:
Jing-shu ZHANG
1
;
Bing-qing CHEN
;
Yan-hui GAO
;
Yan-mei YANG
;
Xuan-lin WANG
;
Yang SONG
;
Wen-guang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cyclooxygenase 1; genetics; metabolism; Cyclooxygenase 2; genetics; metabolism; Enzymes; genetics; metabolism; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; drug effects; Gene Expression Regulation, Neoplastic; drug effects; Humans; Linoleic Acids; metabolism; Linoleic Acids, Conjugated; pharmacology; Lipid Metabolism; drug effects; Lipoxygenase; genetics; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Preventive Medicine 2005;39(3):195-198
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell (SGC-7901).
METHODSSGC-7901 was treated with c9,t11-CLA by 200, 100, 50 or 25 micromol/L for 24 hours. The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Delta6-desaturase, Delta5-desaturase, COX-1, COX-2, 5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSAt a concentration of 200, 100, 50, or 25 micromol/L, c9,t11-CLA suppressed the proliferation of SGC-7901 by 54.3%, 20.5%, 10.5% and 2.93%. The c9,t11-CLA might decrease the expression of COX-2 mRNA, and increase the expression of Delta6-desaturase and COX-1 in SGC-7901, but might not affect Delta5-desaturase and 5-LOX.
CONCLUSIONThe effects of c9,t11-CLA on the COX and Delta6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anti-cancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.
