Determination of fumonisin biosynthesis gene and toxicity of Fusarium moniliforme strains isolated in China.
- Author:
Xiu-mei LIU
1
;
Xiao-ying WANG
;
Mao-feng QIU
;
Xiu-fang LI
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; Food Contamination; analysis; prevention & control; Fumonisins; analysis; metabolism; Fungal Proteins; genetics; metabolism; Fusarium; genetics; isolation & purification; metabolism; Sesamum; microbiology
- From: Chinese Journal of Preventive Medicine 2005;39(4):249-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between fumonisin biosynthesis gene and toxicity of Fusarium moniliforme isolated in China.
METHODSThe toxigenic gene of 29 Fusarium moniliforme isolated from different provinces and varied food samples were determined. Eighteen fum5-positive strains were selected for biosyhesizing fumonisin and determined by high performance liquid chromatography (HPLC).
RESULTSTwenty-six isolates were identified as fum5 gene positive strains. And all of these strains produced FB(1), FB(2) and FB(3). The amount of FB(1), FB(2) and FB(3) was ranging from 0.41-140.20 mg/kg, 0.06-14.30 mg/kg to 0.30-58.00 mg/kg, except one strain produced a lower level of FB(1) only. It wight be the first report showing a high level fumonisin-producing strain isolated from the sesame sample and identified in the world. The amount of FB(1), FB(2) and FB(3) produced by the isolate was 128.84 mg/kg, 11.80 mg/kg and 14.88 mg/kg.
CONCLUSIONSIt should have a close relationship between fumonisins biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. The study demonstrated that strain of Fusarium moniliforme might contaminate the sesame sample and produce a high level of fumonisins.
