Association between genetic polymorphisms of metabolic enzymes and susceptibility of colorectal cancer.
- Author:
Chun-hong FAN
1
;
Ming-juan JIN
;
Yang ZHANG
;
Liang SONG
;
Hong XU
;
Qin-ting JIANG
;
Wei-ping YU
;
Kun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cohort Studies; Colorectal Neoplasms; genetics; Cytochrome P-450 CYP1A1; genetics; Follow-Up Studies; Gene Frequency; Genetic Predisposition to Disease; genetics; Genotype; Glutathione Transferase; genetics; Humans; Logistic Models; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide
- From: Chinese Journal of Preventive Medicine 2006;40(1):13-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between CYP1A1, GSTM1, T1, UGT1A7 polymorphisms and colorectal cancer risk.
METHODSA case-control study of 140 patients with cancers and 343 health controls was conducted to investigate the role of CYP1A1, GSTM1, T1, UGT1A7 polymorphisms in colorectal cancer. Gene-gene interactions among CYP1A1, GSTM1, T1, UGT1A7 polymorphisms were detected by case-control study and case-only study. Genotypes of four genes polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and unconditional logistic regression was adopted to analyze the data.
RESULTSThe CC, TC and CC genotypes of CYP1A1 T6235C significantly decreased the colorectal cancer risk as compared to TT genotype (OR = 0.493, 95% CI: 0.254-0.956, OR = 0.638, 95% CI: 0.427-0.952). GSTM1 and GSTT1 null genotype had no significant association with the increased risk of colorectal cancer while the mutant variants of UGT1A7 might increase the risk of colorectal cancer significantly (OR = 2.501, 95% CI: 1.456-4.296). The CORvalue for the gene-gene interactions between CYP1A1 variant and the null genotype of GSTT1, GSTM1-deleted and GSTT1-deleted genotype in the case-only design were 2.617 (95% CI: 1.015-6.752) and 3.935 (95% CI: 1.323-11.706), respectively. There was no significant interaction between CYP1A1 and GSTM1, CYP1A1 and UGT1A7.
CONCLUSIONThis study suggests that CYP1A1 and UGT1A7 variants might be associated with colorectal cancer. CYP1A1 and GSTM1 might interact on GSTT1 to influence the risk of colorectal cancer.
