Determination of glycosides in traditional Chinese medicine liu-wei di-huang by RP-HPLC.
- Author:
Shan-li XIONG
1
;
Yi-min ZHAO
;
Xin-hui LUAN
;
Shan-yi QIAO
;
Lei SUN
;
Feng-xia REN
;
Ji-fen GUO
;
Yong-xiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Bridged-Ring Compounds; analysis; Chromatography, High Pressure Liquid; methods; Cornus; chemistry; Dioscorea; chemistry; Drug Combinations; Drugs, Chinese Herbal; analysis; chemistry; Glucosides; analysis; Iridoids; analysis; Monoterpenes; Plants, Medicinal; chemistry; Quality Control; Rehmannia; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(8):735-738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang.
METHODThe samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm.
RESULTThree major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%.
CONCLUSIONThe established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.
