Experimental studies on the apoptosis of HL-60 cells induced by Brucea javanica oil emulsion.
- Author:
Fang WANG
1
;
Yu CAO
;
Hong-yan LIU
;
Zhao-di FU
;
Rui HAN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Brucea; chemistry; HL-60 Cells; Humans; Plant Oils; isolation & purification; pharmacology; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(8):759-762
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inducing effect of Brucea javanica on the apoptpsis of HL-60 cells.
METHODHL-60 cells were treated with Brucea javanica 1:100, 1:40, 1:20 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica.
RESULTDNA ladder was seen in the 1:40 group. The apoptosis cell percentages of 1:40 and 1:20 group were 86.8% and 97% respectively. Cells of 1:40 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 1:20 and 1:40 Brucea javanica under electron microscope.
CONCLUSION1:20 and 1:40 Brucea Javanica showed obvious apoptosis inducing effect of HL-60.
