The inhibitive effect of tea polyphenols on oxidized low density lipoprotein intake in cultured rat mesangial cells.
- Author:
Yan ZHANG
1
;
Hua GAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Cholesterol; metabolism; Flavonoids; isolation & purification; pharmacology; Foam Cells; metabolism; Glomerular Mesangium; cytology; metabolism; Lipoproteins, LDL; metabolism; Male; Phenols; isolation & purification; pharmacology; Polyphenols; Rats; Rats, Wistar; Tea; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(8):762-766
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitive effect of tea polyphenols (TP) on oxidized low density lipoprotein (OX-LDL) intake in cultured rat mesangial cells.
METHODRat mesangial cells incubated in 80 mg.L-1 OX-LDL were treated with TP at different concentrations. Total cholesterol (TC) content of the mesangial cells and proportion of mesangial cells containing lipid droplets were measured, and ultrastructure observation was performed.
RESULTFoam cells were observed in mesangial cells stimulated by OX-LDL, and both the TC content and the proportion of lipid-droplet-containing cell were higher in these cells than in mesangial cells cultured in lipid free conditions. There were concentration-dependent decreases both in TC content and lipid-droplet-containing cell proportions when treated with TP from 40 ng.L-1 to 40 mg.L-1. The number of lipid droplets was also decreased in TP treated mesangial cells.
CONCLUSIONTP has an inhibitive effect on OX-LDL intake in cultured rat mesangial cells, which is in a concentration-dependent manner from 40 ng.L-1 to 40 mg.L-1.
