A comparison of the protective activity of liver injury induced by D-galact-osamine between huangqin-tang and their metabolites by human intestinal bacteria.
- Author:
Feng ZUO
1
;
Zhong-ming ZHOU
;
Mei-zhen YAN
;
Yu-lan XIONG
;
Yan-li WAN
;
Hong-yue SONG
;
Zi-lun WU
;
Rong HE
;
Lian-qiang HUI
;
Wen-hua YE
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Bacteria; metabolism; Chemical and Drug Induced Liver Injury; Drugs, Chinese Herbal; isolation & purification; metabolism; pharmacology; Galactosamine; Intestines; microbiology; Liver Diseases; metabolism; Male; Mice; Plants, Medicinal; chemistry; Protective Agents; metabolism; pharmacology
- From: China Journal of Chinese Materia Medica 2003;28(9):842-844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the protective activity of liver injury induced by D-galactosamine (GalN) between Huangqin-Tang and their metabolites by human intestinal bacteria(HIB).
METHODThe liver injuries in conventional and pseudo-germfree mice were induced by GalN. After oral administration of Huangqin-Tang and their metabolites mixtures by HIB, the serum transaminase (ALT and AST) activities were detected.
RESULTIn conventional mice, large and medium doses (20 and 10 g.kg-1) of Huangqin-Tang decoction significantly reduced the increase of serum ALT activity after 18 h GalN treatment. In pseudo-germfree mice, metabolites significantly reduced the ALT levels. However, Huangqing-Tang didn't affect the ALT levels in this kind of mice. To all of the animals, AST levels remained the same after oral Huangqin-tang or their metabolites.
CONCLUSIONThe metabolism by intestinal bacteria plays a role in pharmacological effects of constituents of Chinese herbal medicine. The metabolites of the constituents by intestinal bacteria were the real active components in vivo.
