Saccharide analysis of si-wu-tang by automated multiple development and off-line coupling thin layer chromatography electrospray ionization mass spectrometry techniques.
- Author:
Qian-de LIANG
1
;
Wei-hua LI
;
Hong-xia WANG
;
Sheng-qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; chemistry; Chromatography, Thin Layer; Disaccharides; analysis; Drugs, Chinese Herbal; chemistry; Fructose; analysis; Glucose; analysis; Ligusticum; chemistry; Monosaccharides; analysis; chemistry; Paeonia; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Rehmannia; chemistry; Spectrometry, Mass, Electrospray Ionization; Trisaccharides; analysis
- From: China Journal of Chinese Materia Medica 2003;28(7):632-635
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo make qualitative analysis on saccharide spots in thin layer chromatography (TLC) chromatogram of SI-WU-TANG extract C, which possesses blood-enrichment activity.
METHODTLC chromatogram of SI-WU-TANG extract C was obtained by using Automated Multiple Development (AMD) method. 4 major spots in the chromatogram were analyzed by off-line coupling TLC electrospray ionization mass spectrometry (ESI-MS) technique. Moreover, composition of monosaccharides in the fraction was analyzed by AMD technique.
RESULTMain constituents of substances from the 4 spots were monosaccharide, disaccharide, trisaccharide and tetrasaccharide respectively. Monosaccharide was mainly composed of fructose and glucose.
CONCLUSIONOff-line coupling TLC ESI-MS can simply and rapidly provide qualitative examination of saccharide spots in TLC chromatogram of Traditional Chinese Medicine. AMD method can make good separation of 8 frequently-observed monosaccharides in a regular 10 cm silica gel plate, the process of which was automated, AMD and off-line coupling TLC ESI-MS techniques show good value in saccharides analysis.
