Sbudies on the effect of parthenolide on the proliferation of rat vascular smooth muscle cells and the mechanism.
- Author:
Shao-xiang WENG
1
;
Jiang SHAN
;
Xiao-xia LIN
;
Guo-sheng FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Asteraceae; chemistry; Cell Cycle; drug effects; Cell Division; drug effects; Cells, Cultured; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; metabolism; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-fos; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Rats; Rats, Sprague-Dawley; Sesquiterpenes; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2003;28(7):647-650
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism.
METHODVascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation.
RESULTParthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner.
CONCLUSIONParthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.
