Cochlear mitochondrial DNA3867bp deletion in aged mice.
- Author:
Xinxin ZHANG
1
;
Dongyi HAN
;
Dalian DING
;
Pu DAI
;
Weiyan YANG
;
Sichang JIANG
;
Richard J SALVI
Author Information
- Publication Type:Journal Article
- MeSH: Aging; genetics; Animals; Base Sequence; Cochlea; metabolism; DNA, Mitochondrial; analysis; genetics; Mice; Molecular Sequence Data; Oxidative Phosphorylation; Presbycusis; etiology; Sequence Deletion
- From: Chinese Medical Journal 2002;115(9):1390-1393
- CountryChina
- Language:English
-
Abstract:
OBJECTIVESTo study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.
METHODSWe detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
RESULTSmtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
CONCLUSIONCochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.
