Difference in DNA sequences in SSU rDNA variable regions among pathogens isolated from different epidemic foci of visceral leishmaniasis in China.
- Author:
Xiaosu HU
1
;
Lingyi BU
;
Ying MA
;
Yajing WANG
;
Baoqian JING
;
Taolin YI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA, Protozoan; chemistry; DNA, Ribosomal; chemistry; Humans; Leishmania donovani; genetics; Leishmaniasis, Visceral; parasitology; Point Mutation; Polymerase Chain Reaction
- From: Chinese Medical Journal 2002;115(10):1457-1459
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L.d.) isolates from different epidemic foci in China.
METHODSSpecific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM(R)-T Easy Vectors. After that, the specific fragments were sequenced by an automated DNA sequencer.
RESULTSSequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length. All 5 point mutations were located in two unique sequence blocks (UQ-I and UQ-II), and no insertions or deletions were found. The identities of comparison of Leishmania in GeneBank were more than 98%.
CONCLUSIONFive point mutations exist in the SSU rDNA variable region of 5 L.d. isolates from different epidemic foci of visceral leishmaniasis (VL) in China. Sequence differences of the SSU rDNA variable region exist among L.d. isolates from different foci.
